New England Biolabs    
               
Double Digest Finder (Utilizing old NEB Restriction Enzyme Buffer System)
Cleaving a DNA substrate with two restriction enzymes simultaneously (double digestion) is a common timesaving procedure. Use this tool to select reaction conditions amenable to any two NEB restriction enzymes.
To perform a double digest calculation, please select both 1st and 2nd enzymes.
Select 1st enzyme:       Select 2nd enzyme:       


Click to access the new Double Digest Finder (Utilizing new NEB Restriction Enzyme Buffer System)